Composite

Part:BBa_K1039021:Design

Designed by: Jama Hagi-Yusuf   Group: iGEM13_Waterloo   (2013-09-13)


Bxb1 integrase and Recombination Directionality Factor (RDF) Under “Lock and Key” Control with LacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1660
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
    Illegal BamHI site found at 1934
    Illegal XhoI site found at 2021
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2573
    Illegal NgoMIV site found at 2660
    Illegal AgeI site found at 1710
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2768
    Illegal BsaI.rc site found at 3177


Design Notes

Bxb1 Integrase (BBa_K1039003) has the following three silent mutations, to remove forbidden restriction sites, base 273 G->A, base 1269 A->T and base 1440 G->A



Source

BBa_I714036, BBa_R0011, BBa_J23119 and BBa_Q04121 were obtained from the iGEM 2013 distribution kit. The rest of the biobricks were synthesized by BioBasic Inc.

References